phosphorylated smad2 p smad2 (Cell Signaling Technology Inc)
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Phosphorylated Smad2 P Smad2, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 4488 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 96 stars, based on 4488 article reviews
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other:Article Title: Strontium-containing α-calcium sulfate hemihydrate promotes bone repair via the TGF-β/Smad signaling pathway Article Snippet: After being washed, the membranes were incubated overnight with primary antibodies at 4°C; after which, they were incubated with horseradish peroxidase conjugated donkey-anti-rabbit secondary antibodies at room temperature for 2 h. Immunostaining was detected with the use of enhanced chemiluminescent reagents (P0018FS, Beyotime Institute of Biotechnology). Blocking Assay:Article Title: Repression of Smad4 by MicroRNA-1285 moderates TGF-β-induced epithelial–mesenchymal transition in proliferative vitreoretinopathy Article Snippet: Specifically, 20 μg of protein in each sample was loaded and subjected to sodium dodecyl sulfate–polyacrylamide gel electrophoresis and then transferred to a polyvinylidene fluoride membrane (Millipore, Billerica, MA, USA). .. After blocking with 5% skimmed milk dissolved in a phosphate buffer solution with Tween20 at room temperature for 1hour, the membranes were directly probed with primary antibodies againstα-smooth muscle actin (α-SMA; Sigma-Aldrich, St. Louis, MO, USA), anti-zonula occludens (ZO)-1 (Zymed Laboratories, South San Francisco, CA, USA), Article Title: HaCaT-conditioned medium supplemented with the small molecule inhibitors SB431542 and CHIR99021 and the growth factor PDGF-AA prevents the dedifferentiation of dermal papilla cells in vitro Article Snippet: .. After blocking in sterile PBS containing 5% non-fat dry skimmed milk and 0.05% (v/v) Tween-20 at room temperature for 1 h, the blots were incubated with the following primary antibodies overnight at 4°C: Sox2 (cat. no. 3579; 1:1,000; Cell Signaling Technology, Inc.), ALP (cat. no. ab108337; 1:2,000; Abcam), Versican (cat. no. ab19345; 1:1,000; Abcam), Incubation:Article Title: Repression of Smad4 by MicroRNA-1285 moderates TGF-β-induced epithelial–mesenchymal transition in proliferative vitreoretinopathy Article Snippet: Specifically, 20 μg of protein in each sample was loaded and subjected to sodium dodecyl sulfate–polyacrylamide gel electrophoresis and then transferred to a polyvinylidene fluoride membrane (Millipore, Billerica, MA, USA). .. After blocking with 5% skimmed milk dissolved in a phosphate buffer solution with Tween20 at room temperature for 1hour, the membranes were directly probed with primary antibodies againstα-smooth muscle actin (α-SMA; Sigma-Aldrich, St. Louis, MO, USA), anti-zonula occludens (ZO)-1 (Zymed Laboratories, South San Francisco, CA, USA), Article Title: HaCaT-conditioned medium supplemented with the small molecule inhibitors SB431542 and CHIR99021 and the growth factor PDGF-AA prevents the dedifferentiation of dermal papilla cells in vitro Article Snippet: .. After blocking in sterile PBS containing 5% non-fat dry skimmed milk and 0.05% (v/v) Tween-20 at room temperature for 1 h, the blots were incubated with the following primary antibodies overnight at 4°C: Sox2 (cat. no. 3579; 1:1,000; Cell Signaling Technology, Inc.), ALP (cat. no. ab108337; 1:2,000; Abcam), Versican (cat. no. ab19345; 1:1,000; Abcam), Article Title: Selective blocking of CXCR2 prevents and reverses atrial fibrillation in spontaneously hypertensive rats. Article Snippet: .. Proteins (40-60 μg) were subjected to sodium dodecyl sulphate polyacrylamide gel electrophoresis and transferred to a polyvinylidene difluoride membrane (Bio-Rad), which was incubated with primary antibodies against CXCR2, TGF-β1, Smad2/3, Sterility:Article Title: HaCaT-conditioned medium supplemented with the small molecule inhibitors SB431542 and CHIR99021 and the growth factor PDGF-AA prevents the dedifferentiation of dermal papilla cells in vitro Article Snippet: .. After blocking in sterile PBS containing 5% non-fat dry skimmed milk and 0.05% (v/v) Tween-20 at room temperature for 1 h, the blots were incubated with the following primary antibodies overnight at 4°C: Sox2 (cat. no. 3579; 1:1,000; Cell Signaling Technology, Inc.), ALP (cat. no. ab108337; 1:2,000; Abcam), Versican (cat. no. ab19345; 1:1,000; Abcam), Cell-Signaling:Article Title: HaCaT-conditioned medium supplemented with the small molecule inhibitors SB431542 and CHIR99021 and the growth factor PDGF-AA prevents the dedifferentiation of dermal papilla cells in vitro Article Snippet: .. After blocking in sterile PBS containing 5% non-fat dry skimmed milk and 0.05% (v/v) Tween-20 at room temperature for 1 h, the blots were incubated with the following primary antibodies overnight at 4°C: Sox2 (cat. no. 3579; 1:1,000; Cell Signaling Technology, Inc.), ALP (cat. no. ab108337; 1:2,000; Abcam), Versican (cat. no. ab19345; 1:1,000; Abcam), Article Title: Inhibitory effects of umbelliferone on carbon tetrachloride-induced hepatic fibrosis in rats through the TGF‑β1‑Smad signaling pathway Article Snippet: The bicinchoninic acid (BCA) assay kit was from Thermo Fisher Scientific, Inc. .. The primary antibodies against TGF-β1, α-SMA, ALP Assay:Article Title: HaCaT-conditioned medium supplemented with the small molecule inhibitors SB431542 and CHIR99021 and the growth factor PDGF-AA prevents the dedifferentiation of dermal papilla cells in vitro Article Snippet: .. After blocking in sterile PBS containing 5% non-fat dry skimmed milk and 0.05% (v/v) Tween-20 at room temperature for 1 h, the blots were incubated with the following primary antibodies overnight at 4°C: Sox2 (cat. no. 3579; 1:1,000; Cell Signaling Technology, Inc.), ALP (cat. no. ab108337; 1:2,000; Abcam), Versican (cat. no. ab19345; 1:1,000; Abcam), Polyacrylamide Gel Electrophoresis:Article Title: Selective blocking of CXCR2 prevents and reverses atrial fibrillation in spontaneously hypertensive rats. Article Snippet: .. Proteins (40-60 μg) were subjected to sodium dodecyl sulphate polyacrylamide gel electrophoresis and transferred to a polyvinylidene difluoride membrane (Bio-Rad), which was incubated with primary antibodies against CXCR2, TGF-β1, Smad2/3, Membrane:Article Title: Selective blocking of CXCR2 prevents and reverses atrial fibrillation in spontaneously hypertensive rats. Article Snippet: .. Proteins (40-60 μg) were subjected to sodium dodecyl sulphate polyacrylamide gel electrophoresis and transferred to a polyvinylidene difluoride membrane (Bio-Rad), which was incubated with primary antibodies against CXCR2, TGF-β1, Smad2/3, |
