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phosphorylated smad2 p smad2  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc phosphorylated smad2 p smad2
    Phosphorylated Smad2 P Smad2, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 4488 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/phosphorylated+p+smad2+3/Smad2%2F3+Antibody/pmc12570780-88-23-28
    Average 96 stars, based on 4488 article reviews
    phosphorylated smad2 p smad2 - by Bioz Stars, 2026-10
    96/100 stars

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    other:

    Article Title: Strontium-containing α-calcium sulfate hemihydrate promotes bone repair via the TGF-β/Smad signaling pathway
    Article Snippet: After being washed, the membranes were incubated overnight with primary antibodies at 4°C; after which, they were incubated with horseradish peroxidase conjugated donkey-anti-rabbit secondary antibodies at room temperature for 2 h. Immunostaining was detected with the use of enhanced chemiluminescent reagents (P0018FS, Beyotime Institute of Biotechnology).

    Blocking Assay:

    Article Title: Repression of Smad4 by MicroRNA-1285 moderates TGF-β-induced epithelial–mesenchymal transition in proliferative vitreoretinopathy
    Article Snippet: Specifically, 20 μg of protein in each sample was loaded and subjected to sodium dodecyl sulfate–polyacrylamide gel electrophoresis and then transferred to a polyvinylidene fluoride membrane (Millipore, Billerica, MA, USA). .. After blocking with 5% skimmed milk dissolved in a phosphate buffer solution with Tween20 at room temperature for 1hour, the membranes were directly probed with primary antibodies againstα-smooth muscle actin (α-SMA; Sigma-Aldrich, St. Louis, MO, USA), anti-zonula occludens (ZO)-1 (Zymed Laboratories, South San Francisco, CA, USA), phosphorylated (p)-Smad2/3 (Cell Signaling), Smad4(1:1000 dilution for all), and glyceraldehyde 3-phosphate dehydrogenase (GAPDH; 1:25,000dilution) overnight at 4°C and then incubated with the horseradish peroxidase–conjugated secondary antibody (Jackson Immuno Research Laboratories, West Grove, PA, USA) for 1 hour. .. Next, the proteins were visualized using enhanced chemiluminescence (ECL; Millipore).

    Article Title: HaCaT-conditioned medium supplemented with the small molecule inhibitors SB431542 and CHIR99021 and the growth factor PDGF-AA prevents the dedifferentiation of dermal papilla cells in vitro
    Article Snippet: .. After blocking in sterile PBS containing 5% non-fat dry skimmed milk and 0.05% (v/v) Tween-20 at room temperature for 1 h, the blots were incubated with the following primary antibodies overnight at 4°C: Sox2 (cat. no. 3579; 1:1,000; Cell Signaling Technology, Inc.), ALP (cat. no. ab108337; 1:2,000; Abcam), Versican (cat. no. ab19345; 1:1,000; Abcam), phosphorylated (p)-Smad2/3 (cat. no. 8828; 1:1,000; Cell Signaling Technology, Inc.), Smad2/3 (cat. no. 5678; 1:1,000; Cell Signaling Technology, Inc.), β-catenin (cat. no. 8480; 1:1,000; Cell Signaling Technology, Inc.), lymphoid enhancer-binding factor 1 (Lef-1) (cat. no. 2230; 1:1,000; Cell Signaling Technology, Inc.) and β-actin (cat. no. AT0001; 1:1,000; Engibody Biotechnology, Inc.). .. Horseradish peroxidase-conjugated anti-mouse (cat. no. 7076; 1:1,000; Cell Signaling Technology, Inc.) and anti-rabbit (cat. no. 7074; 1:1,000; Cell Signaling Technology, Inc.) antibodies were used as secondary antibodies at room temperature for 1 h. The immune complexes were assayed with an enhanced chemiluminescence kit (Invitrogen; Thermo Fisher Scientific, Inc.) and the blots were analyzed with densitometry using ImageJ 1.53a software (National Institutes of Health).

    Incubation:

    Article Title: Repression of Smad4 by MicroRNA-1285 moderates TGF-β-induced epithelial–mesenchymal transition in proliferative vitreoretinopathy
    Article Snippet: Specifically, 20 μg of protein in each sample was loaded and subjected to sodium dodecyl sulfate–polyacrylamide gel electrophoresis and then transferred to a polyvinylidene fluoride membrane (Millipore, Billerica, MA, USA). .. After blocking with 5% skimmed milk dissolved in a phosphate buffer solution with Tween20 at room temperature for 1hour, the membranes were directly probed with primary antibodies againstα-smooth muscle actin (α-SMA; Sigma-Aldrich, St. Louis, MO, USA), anti-zonula occludens (ZO)-1 (Zymed Laboratories, South San Francisco, CA, USA), phosphorylated (p)-Smad2/3 (Cell Signaling), Smad4(1:1000 dilution for all), and glyceraldehyde 3-phosphate dehydrogenase (GAPDH; 1:25,000dilution) overnight at 4°C and then incubated with the horseradish peroxidase–conjugated secondary antibody (Jackson Immuno Research Laboratories, West Grove, PA, USA) for 1 hour. .. Next, the proteins were visualized using enhanced chemiluminescence (ECL; Millipore).

    Article Title: HaCaT-conditioned medium supplemented with the small molecule inhibitors SB431542 and CHIR99021 and the growth factor PDGF-AA prevents the dedifferentiation of dermal papilla cells in vitro
    Article Snippet: .. After blocking in sterile PBS containing 5% non-fat dry skimmed milk and 0.05% (v/v) Tween-20 at room temperature for 1 h, the blots were incubated with the following primary antibodies overnight at 4°C: Sox2 (cat. no. 3579; 1:1,000; Cell Signaling Technology, Inc.), ALP (cat. no. ab108337; 1:2,000; Abcam), Versican (cat. no. ab19345; 1:1,000; Abcam), phosphorylated (p)-Smad2/3 (cat. no. 8828; 1:1,000; Cell Signaling Technology, Inc.), Smad2/3 (cat. no. 5678; 1:1,000; Cell Signaling Technology, Inc.), β-catenin (cat. no. 8480; 1:1,000; Cell Signaling Technology, Inc.), lymphoid enhancer-binding factor 1 (Lef-1) (cat. no. 2230; 1:1,000; Cell Signaling Technology, Inc.) and β-actin (cat. no. AT0001; 1:1,000; Engibody Biotechnology, Inc.). .. Horseradish peroxidase-conjugated anti-mouse (cat. no. 7076; 1:1,000; Cell Signaling Technology, Inc.) and anti-rabbit (cat. no. 7074; 1:1,000; Cell Signaling Technology, Inc.) antibodies were used as secondary antibodies at room temperature for 1 h. The immune complexes were assayed with an enhanced chemiluminescence kit (Invitrogen; Thermo Fisher Scientific, Inc.) and the blots were analyzed with densitometry using ImageJ 1.53a software (National Institutes of Health).

    Article Title: Selective blocking of CXCR2 prevents and reverses atrial fibrillation in spontaneously hypertensive rats.
    Article Snippet: .. Proteins (40-60 μg) were subjected to sodium dodecyl sulphate polyacrylamide gel electrophoresis and transferred to a polyvinylidene difluoride membrane (Bio-Rad), which was incubated with primary antibodies against CXCR2, TGF-β1, Smad2/3, phosphorylated (p)-Smad2/3, P65, p-P65, NOX1 and NOX2 (Cell Signaling Technologies, Boston, MA, USA), and GAPDH (Proteintech Group. .. Inc, Rosemont, IL, USA).14,17 Antimouse or anti-rabbit IgG secondary antibodies were purchased from Cell Signaling Technologies.

    Sterility:

    Article Title: HaCaT-conditioned medium supplemented with the small molecule inhibitors SB431542 and CHIR99021 and the growth factor PDGF-AA prevents the dedifferentiation of dermal papilla cells in vitro
    Article Snippet: .. After blocking in sterile PBS containing 5% non-fat dry skimmed milk and 0.05% (v/v) Tween-20 at room temperature for 1 h, the blots were incubated with the following primary antibodies overnight at 4°C: Sox2 (cat. no. 3579; 1:1,000; Cell Signaling Technology, Inc.), ALP (cat. no. ab108337; 1:2,000; Abcam), Versican (cat. no. ab19345; 1:1,000; Abcam), phosphorylated (p)-Smad2/3 (cat. no. 8828; 1:1,000; Cell Signaling Technology, Inc.), Smad2/3 (cat. no. 5678; 1:1,000; Cell Signaling Technology, Inc.), β-catenin (cat. no. 8480; 1:1,000; Cell Signaling Technology, Inc.), lymphoid enhancer-binding factor 1 (Lef-1) (cat. no. 2230; 1:1,000; Cell Signaling Technology, Inc.) and β-actin (cat. no. AT0001; 1:1,000; Engibody Biotechnology, Inc.). .. Horseradish peroxidase-conjugated anti-mouse (cat. no. 7076; 1:1,000; Cell Signaling Technology, Inc.) and anti-rabbit (cat. no. 7074; 1:1,000; Cell Signaling Technology, Inc.) antibodies were used as secondary antibodies at room temperature for 1 h. The immune complexes were assayed with an enhanced chemiluminescence kit (Invitrogen; Thermo Fisher Scientific, Inc.) and the blots were analyzed with densitometry using ImageJ 1.53a software (National Institutes of Health).

    Cell-Signaling:

    Article Title: HaCaT-conditioned medium supplemented with the small molecule inhibitors SB431542 and CHIR99021 and the growth factor PDGF-AA prevents the dedifferentiation of dermal papilla cells in vitro
    Article Snippet: .. After blocking in sterile PBS containing 5% non-fat dry skimmed milk and 0.05% (v/v) Tween-20 at room temperature for 1 h, the blots were incubated with the following primary antibodies overnight at 4°C: Sox2 (cat. no. 3579; 1:1,000; Cell Signaling Technology, Inc.), ALP (cat. no. ab108337; 1:2,000; Abcam), Versican (cat. no. ab19345; 1:1,000; Abcam), phosphorylated (p)-Smad2/3 (cat. no. 8828; 1:1,000; Cell Signaling Technology, Inc.), Smad2/3 (cat. no. 5678; 1:1,000; Cell Signaling Technology, Inc.), β-catenin (cat. no. 8480; 1:1,000; Cell Signaling Technology, Inc.), lymphoid enhancer-binding factor 1 (Lef-1) (cat. no. 2230; 1:1,000; Cell Signaling Technology, Inc.) and β-actin (cat. no. AT0001; 1:1,000; Engibody Biotechnology, Inc.). .. Horseradish peroxidase-conjugated anti-mouse (cat. no. 7076; 1:1,000; Cell Signaling Technology, Inc.) and anti-rabbit (cat. no. 7074; 1:1,000; Cell Signaling Technology, Inc.) antibodies were used as secondary antibodies at room temperature for 1 h. The immune complexes were assayed with an enhanced chemiluminescence kit (Invitrogen; Thermo Fisher Scientific, Inc.) and the blots were analyzed with densitometry using ImageJ 1.53a software (National Institutes of Health).

    Article Title: Inhibitory effects of umbelliferone on carbon tetrachloride-induced hepatic fibrosis in rats through the TGF‑β1‑Smad signaling pathway
    Article Snippet: The bicinchoninic acid (BCA) assay kit was from Thermo Fisher Scientific, Inc. .. The primary antibodies against TGF-β1, α-SMA, phosphorylated (p)-Smad2/3, Smad2/3 and β-actin and the horseradish peroxidase (HRP)-conjugated rabbit anti-mouse IgG secondary antibodies were from Cell Signaling Technology, Inc. .. The ECL chemiluminescence kit was from Beyotime Institute of Biotechnology.

    ALP Assay:

    Article Title: HaCaT-conditioned medium supplemented with the small molecule inhibitors SB431542 and CHIR99021 and the growth factor PDGF-AA prevents the dedifferentiation of dermal papilla cells in vitro
    Article Snippet: .. After blocking in sterile PBS containing 5% non-fat dry skimmed milk and 0.05% (v/v) Tween-20 at room temperature for 1 h, the blots were incubated with the following primary antibodies overnight at 4°C: Sox2 (cat. no. 3579; 1:1,000; Cell Signaling Technology, Inc.), ALP (cat. no. ab108337; 1:2,000; Abcam), Versican (cat. no. ab19345; 1:1,000; Abcam), phosphorylated (p)-Smad2/3 (cat. no. 8828; 1:1,000; Cell Signaling Technology, Inc.), Smad2/3 (cat. no. 5678; 1:1,000; Cell Signaling Technology, Inc.), β-catenin (cat. no. 8480; 1:1,000; Cell Signaling Technology, Inc.), lymphoid enhancer-binding factor 1 (Lef-1) (cat. no. 2230; 1:1,000; Cell Signaling Technology, Inc.) and β-actin (cat. no. AT0001; 1:1,000; Engibody Biotechnology, Inc.). .. Horseradish peroxidase-conjugated anti-mouse (cat. no. 7076; 1:1,000; Cell Signaling Technology, Inc.) and anti-rabbit (cat. no. 7074; 1:1,000; Cell Signaling Technology, Inc.) antibodies were used as secondary antibodies at room temperature for 1 h. The immune complexes were assayed with an enhanced chemiluminescence kit (Invitrogen; Thermo Fisher Scientific, Inc.) and the blots were analyzed with densitometry using ImageJ 1.53a software (National Institutes of Health).

    Polyacrylamide Gel Electrophoresis:

    Article Title: Selective blocking of CXCR2 prevents and reverses atrial fibrillation in spontaneously hypertensive rats.
    Article Snippet: .. Proteins (40-60 μg) were subjected to sodium dodecyl sulphate polyacrylamide gel electrophoresis and transferred to a polyvinylidene difluoride membrane (Bio-Rad), which was incubated with primary antibodies against CXCR2, TGF-β1, Smad2/3, phosphorylated (p)-Smad2/3, P65, p-P65, NOX1 and NOX2 (Cell Signaling Technologies, Boston, MA, USA), and GAPDH (Proteintech Group. .. Inc, Rosemont, IL, USA).14,17 Antimouse or anti-rabbit IgG secondary antibodies were purchased from Cell Signaling Technologies.

    Membrane:

    Article Title: Selective blocking of CXCR2 prevents and reverses atrial fibrillation in spontaneously hypertensive rats.
    Article Snippet: .. Proteins (40-60 μg) were subjected to sodium dodecyl sulphate polyacrylamide gel electrophoresis and transferred to a polyvinylidene difluoride membrane (Bio-Rad), which was incubated with primary antibodies against CXCR2, TGF-β1, Smad2/3, phosphorylated (p)-Smad2/3, P65, p-P65, NOX1 and NOX2 (Cell Signaling Technologies, Boston, MA, USA), and GAPDH (Proteintech Group. .. Inc, Rosemont, IL, USA).14,17 Antimouse or anti-rabbit IgG secondary antibodies were purchased from Cell Signaling Technologies.



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    Fig. 7. Histological changes and modulation of the TGF-β/SMAD/COL1A1 signaling pathway in a bleomycin-Induced IPF model in C57/BL6 mice. (A) The histo logical changes measured by H&E staining in lung tissue of bleomycin-induced IPF mice. Scale bars, 300 μm. (B-F) Expression and ratio of TGF-β/GAPDH, <t>p-SMAD2/</t> SMAD2, p-SMAD3/SMAD3, COL1A1/GAPDH and Caveolin-1/GAPDH were investigated using western blot analysis. Statistical analysis was performed using one- way ANOVA. *p<0.05, ***p<0.001 versus the BLM.
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    Fig. 7. Histological changes and modulation of the TGF-β/SMAD/COL1A1 signaling pathway in a bleomycin-Induced IPF model in C57/BL6 mice. (A) The histo logical changes measured by H&E staining in lung tissue of bleomycin-induced IPF mice. Scale bars, 300 μm. (B-F) Expression and ratio of TGF-β/GAPDH, p-SMAD2/ SMAD2, p-SMAD3/SMAD3, COL1A1/GAPDH and Caveolin-1/GAPDH were investigated using western blot analysis. Statistical analysis was performed using one- way ANOVA. *p<0.05, ***p<0.001 versus the BLM.

    Journal: Ecotoxicology and environmental safety

    Article Title: A novel method for real-time inhalation toxicity assessment in mice using respirometric system: A promising tool for respiratory toxicology.

    doi: 10.1016/j.ecoenv.2024.117333

    Figure Lengend Snippet: Fig. 7. Histological changes and modulation of the TGF-β/SMAD/COL1A1 signaling pathway in a bleomycin-Induced IPF model in C57/BL6 mice. (A) The histo logical changes measured by H&E staining in lung tissue of bleomycin-induced IPF mice. Scale bars, 300 μm. (B-F) Expression and ratio of TGF-β/GAPDH, p-SMAD2/ SMAD2, p-SMAD3/SMAD3, COL1A1/GAPDH and Caveolin-1/GAPDH were investigated using western blot analysis. Statistical analysis was performed using one- way ANOVA. *p<0.05, ***p<0.001 versus the BLM.

    Article Snippet: Anti-TGF-β, anti-SMAD2, anti-phosphorylated SMAD2 (p-SMAD2), anti-SMAD3, anti-phosphorylated SMAD3 (pSMAD3), anti-Caveolin-1, and anti-GAPDH antibodies were purchased from Cell Signaling Technology Inc. (Danvers, MA).

    Techniques: Staining, Expressing, Western Blot